None of these ligands activated CquiOR161·CquiOrco-expressing oocytes. As a positive control, CquiOR1·CquiOrco-expressing oocytes in the UM laboratory gave medium to large responses when challenged

with indole, 4-ethylphenol, 4-methylphenol, phenol, acetophenone, benzaldehyde, and 6-methyl-5-hepten-2-one. Although we cannot rule out the possibility that we did not challenge CquiOR161 with the right ligand, this seems unlikely as in both labs we subjected oocytes expressing the receptor to all currently known odorants with physiological and/or ecological significance in Culex mosquitoes. In conclusion, we have cloned four ORs, which are enriched in female mosquito antennae. Despite several attempts, one of them, CquiOR161,

was Nutlin3a silent as it did not respond to any of ligands tested. By contrast, CquiOR1 showed behavior NVP-BKM120 mw of a generalist OR as it responded to various compounds, including alcohols and ketones of biological significance. Another OR, CquiOR73, was more tuned to phenolic compounds, with eugenol, which is the major constituent of clover oil and has mosquito repellent activity, being the best ligand. Lastly, CquiOR44 showed robust responses only to plant-derived terpenoid compound, particularly fenchone. The newly de-orphanized ORs might be involved Y-27632 nmr in the detection of plant-derived kairomones and/or repellents. Research reported in this publication was supported by the National Institutes of Health under awards R01AI095514 from the National Institute of Allergy and Infectious Diseases (to W.S.L.) and RO1DC011091 from the National Institute on Deafness and Other Communicative Disorders (to C.W.L.). The content is solely the responsibility

of the authors and does not necessarily represent the official views of NIH. F.R.S. (Universidade de São Paulo, Campus of Piracicaba) received an undergraduate scholarship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) under a FIPSE-CAPSE sponsored US-Brazil Higher education Consortium Program. FZ sabbatical leave at UC Davis was supported in part by the China Scholarship Council. “
“The authors regret “Table1. Results of hierarchical partitioning for the effect of climatic factors on soil, and the effect of climatic factors and Mg available on leaf, acorn and weevilMg” is wrong, and it should be “The results of hierarchical partitioning for the effect of climatic factors and acorn elements on the weevil larva stoichiometric composition and lipid”. The authors would like to apologise for any inconvenience caused. “
“Pre-oral digestion is described as the liquefaction of the solid tissues of the prey caused by secretions of the predator.