4%) in this trial was lower than the reported rates in multiple recent large prospective randomized and cohort comparison studies in the literature (3% to 15%).7, 8, 10 and 11 The primary objective was to demonstrate the safety and feasibility of the intraoperative assessment of colon and rectal perfusion using fluorescence angiography during left colectomies and low anterior resections. This technology was easy to implement because the device is similar to a standard laparoscope, with a minimal learning curve for application and use. This technology was used by 11

institutions according to surgeon preference, and there were no reported difficulties in assessment despite the absence of any “run in” or practice cases. No MAPK Inhibitor Library cell line complications attributable to the use of the ICG or the device RG7420 supplier were observed. Successful imaging demonstrated no apparent limitation with regard to imaging converted cases. There were no reported limitations

to imaging and/or interpretation with regard to patient comorbidities. Fluorescence angiography has been found to be beneficial in assessing perfusion in earlier reports, aiding in surgical decision making and improving outcomes in cardiothoracic, hepatobiliary, colorectal, foregut, transplant, and plastic surgery.1, 5, 20, 21, 22, 23, 24, 25 and 26 The feasibility and applicability of this new technology with the implications of potentially reducing anastomotic leak rates could make it an invaluable tool for use in high-risk colorectal resections.28 Our results indicate that assessment of microperfusion

of the transected bowel and planned site of anastomosis was associated with revision of surgical plan in nearly 8% of patients. To our knowledge, there are only 2 studies in the literature that have demonstrated the benefits of angiography in colorectal surgery.1 and 5 Kudszus and colleagues5 reported a 14% (n = 201) change in resection margin using laser fluorescence angiography. These findings were confirmed by Jafari and colleagues1 using Firefly (Intuitive Surgical Inc). The authors demonstrated a 19% change in transection point using fluorescence angiography compared with 4.5% using visible or white light during robotic low anterior resections. Our data confirm Tau-protein kinase earlier reports that conventional methods of assessing bowel perfusion are not entirely reliable.1, 5 and 30 To date, subjective methods such as active bleeding, palpable pulsation in the mesentery, and bowel discoloration, have been used. These methods are not objective and can be lacking in a laparoscopic colon resection secondary to the lack of tactile sensation and change in visual cues. In the majority of laparoscopic colectomies, as opposed to open technique, the bowel is transected and reanastomosed shortly after transection of the mesentery, thereby limiting observation time. Conventional techniques of assessing perfusion may not be entirely applicable with laparoscopy and even with laparotomy are not objective.