Aqueous two-phase systems (ATPS) are vital techniques used for the extraction, or even purification, of several compounds and biomolecules, due to their versatility, high effectiveness, high yield, selectivity, low cost and technological simplicity, as well as improved degree of purification. Moreover, ATPS usually allow the combination

SKI-606 of the recovery and purification steps (Cláudio et al., 2010 and Malpiedi et al., 2009). ATPS are generally described as two aqueous liquid phases that are immiscible at critical concentrations of the phase forming components. In the past decades, these systems have been widely applied in the separation/purification of proteins, enzymes, antibiotics, among other biomolecules of interest (Albertsson, 1986, Lima et al., 2002 and Wang et al., 2010). To promote the formation of ATPS, several compounds can be used, such as different polymers (Azevedo et al., 2009 and Silva and Meirelles, 2000), inorganic salts (Lima et al., 2002, Silva et al., 2009 and Souza et al., 2010), sugars (Chen et al., 2010, Wu et al.,

2008 and Wu NVP-AUY922 cell line et al., 2008), and more recently, ionic liquids (Freire et al., 2012, Gutowski et al., 2003, Neves et al., 2009 and Ventura et al., 2009). However, several of these ATPS forming components present some crucial disadvantages, when the objective is to apply them as separation systems for products that Arachidonate 15-lipoxygenase can easily suffer irreversible chemical alterations, and thus lose their main characteristics (for example, their antioxidant capacity). The high viscosity of polymer-based systems, the low window of potential ATPS based in sugars, and the high cost of ionic liquids, are some of the additional disadvantages that should be taken into account for a number of ATPS (Ooi et al., 2009). Thus, in this work, the use of polar hydroalcoholic ATPS was considered (Broinizi et al., 2007, Roesler et al., 2007 and Wang et al., 2010). These systems have already shown to be successful in the separation of enzymes, antibiotics, and

nucleic acids (Broinizi et al., 2007 and Louwrier, 1999). In this work, four alcohols (methanol, ethanol, 1-propanol and 2-propanol) and three salting-out ionic species (K3PO4, K2HPO4 and KH2PO4/K2HPO4) were studied through the formation of ATPS. Their phase diagrams, tie-lines and tie-line lengths were determined at 298 K and atmospheric pressure. Subsequently, these systems were evaluated toward their application as liquid–liquid separation processes for two antioxidants: vanillin and l-ascorbic acid. Both model systems and food waste materials were employed. The results gathered highlight a selective partitioning of both antioxidants, while maintaining their main chemical characteristics as unchanged.