Interestingly, a second CRP determination obtained ∼6 weeks later in the validation cohort, and therefore post-TACE in most cases, retained the

predictive value. The mean CRP levels increased with increasing Barcelona Clinic Liver Cancer (BCLC) stage. CRP levels also stratified patients within the same BCLC stage into long- and short-term survivors. Within BCLC stage B and C, patients with elevated CRP had a shorter survival than patients with low CRP. Within the BCLC C stage, Child B patients with a normal CRP had a survival comparable to Child A patients with an elevated CRP (median survival 15 and 14 months, respectively). Peck-Radosavljevic and co-workers further considered the learn more presence/absence

of clinically evident infection. When unrelated Fulvestrant price to infection, elevated CRP levels correlated directly with tumor characteristics, in particular with tumor burden. This suggests that elevation of CRP might be tumor-related. The authors also compared the proportion of patients with CRP elevation from unknown origin and from infectious origin, in their cohort as well as in a group of 104 non-HCC patients undergoing a transjugular intrahepatic portosystemic shunt (TIPS) placement. Elevated CRP was more frequently of unknown origin in HCC patients than in non-HCC patients (38% versus 17%). Nevertheless, elevated CRP in both groups was associated with poor prognosis. Similarly, Cervoni et al.12 recently reported a benefit of CRP determination in predicting short-term mortality in patients with advanced cirrhosis. What pathophysiological mechanisms trigger CRP elevation in HCC? Low-grade inflammation promotes tumor development. Mdr2−/− mice show defective biliary secretion of phospholipids, spontaneous cholangitis, and eventually develop HCC.13 Transgenic mice that express lymphotoxin α:β develop a chronic parenchymal inflammation with the production of cytokines and eventually HCC.14 IL-6, which is the principal regulator of CRP production, is produced by Kupffer

cells. In the diethylnitrosamine (DEN) rodent model for HCC, IL-6 rises in response to IL-1a, which is released from necrotic hepatocytes.15 This IL-6 production is gender-specific and may partly Digestive enzyme explain the male predominance of HCC.16 In fact, IL-6 expression is elevated in patients with cirrhosis and HCC.17 Moreover IL-6 levels were reported to correlate with the development of HCC.18, 19 This suggests that the hepatocellular signaling pathway of IL-6 might be a therapeutic target. The transcription factor STAT3 mediates the IL-6 effects. STAT3 was found to be activated in the majority of HCCs and seems to identify more aggressive tumors.20 Inactivation of the negative feedback loop of the JAK/STAT pathway by methylation of the SCOCS genes is frequent in HCC.