The latter term has a child “”GO ID 0075073 autophagy of symbiont cells Temsirolimus solubility dmso on or near host surface”", which itself has a lower level child “”GO

ID 0075074 spore autophagy during appressorium formation on or near host”" (see details in Figure 3). The six autophagy-related GO terms are applicable to describe the functions of several genes in fungal pathogens during symbiotic interaction. For example, formation of a functional appressorium in the rice blast fungus requires autophagic cell death of the conidium, which is controlled by the MgATG8 gene. Deletion of MgATG8 results in impaired autophagy, arrested conidial cell death, and a nonpathogenic fungus [14]. Thus, MgATG8 can be annotated with the new term “”GO ID 0075074 spore autophagy during appressorium formation on or near host”". Conclusion Two hundred fifty-six new GO terms were developed to annotate genes or gene Nutlin-3a molecular weight products involved in common pathogenic processes in fungi and oomycetes, including spore dispersal, host

adhesion, recognition, penetration, and invasive growth. These new GO terms provide the opportunity to apply a standard set of terms to annotate gene products of fungi, oomycetes, and their associated hosts, as well as those of other plant-associated pathogens and their hosts. The ability to compare and contrast these annotations for widely different plant-associated microbes and their hosts, using a standardized vocabulary, will greatly facilitate the identification of unique and conserved features of pathogenesis across different kingdoms. In addition, such comparisons should provide insight into the evolution of pathogenic processes. Acknowledgements All authors read and approved the final manuscript. We thank Candace Collmer, Michelle Gwinn Giglio, and the editor at The Gene Ontology Consortium Jane Lomax for their comments and suggestions in developing these PAMGO terms. This work is a part of PAMGO project, which is supported by the USDA NRI-CSREES

(grant number 2005-35600-16370), and the National Science Foundation (grant number EF-0523736). This article has been published as part of BMC Microbiology Volume 9 Supplement 1, 2009: The PAMGO Consortium: Unifying Themes In Microbe-Host Associations Identified Through The STK38 Gene Ontology. The full contents of the supplement are available online at http://​www.​biomedcentral.​com/​1471-2180/​9?​issue=​S1. References 1. Money NP: Why PF-2341066 oomycetes have not stopped being fungi. Mycology Research 1998,102(6):767–768.CrossRef 2. Latijnhouwers M, Wit PJGMD, Govers F: Oomycetes and fungi: similar weaponry to attack plants. Trends in Microbiology 2003,11(10):462–469.CrossRefPubMed 3. Epstein L, Nicholson RL: Adhesion and adhesives of fungi and oomycetes. Biological Adhesives (Edited by: Smith AM, Callow JA). Springer-Verlag Berlin Heidelberg 2006. 4.