Termination of animals was performed 48 hrs after induction of sepsis in order to monitor splenocyte proliferation ((3)H-thymidine incorporation assay), splenocyte apoptosis (Annexin V binding capacity), and cytokine release (IL-1 beta and IL-6, ELISA). Results: Subcutaneous DHEA administration improved the survival rate of septic mice 48 hrs after induction of CLP (75% vs. 47%). This effect was paralleled by a restoration of splenocyte proliferation, a decreased cellular apoptosis rate of splenocytes, and an attenuation of pro-inflammatory cytokine release. In contrast, no significant

effects on the survival rate or cellular immune functions were observed following intravenous or intraperitoneal DHEA administration. LY2835219 mw Conclusions: Subcutaneous administration of DHEA induced an increased survival rate and improved cellular immune functions in septic mice. In contrast, no comparable effects were noticed following intravenous or intraperitoneal administration of DHEA.”
“Magnetic properties of Fe nanoparticles KPT-8602 molecular weight with different sizes synthesized by a physical deposition technique have been investigated experimentally. We have used a high pressure sputtering technique to deposit iron nanoparticles on a silicon substrate. The nanoparticles are then analyzed using atomic force microscopy (AFM), transmission electron microscopy (TEM), and superconducting quantum interference device

techniques. TEM and AFM data show that the particle size could be tuned by adjusting the deposition conditions. The magnetic properties have been investigated from temperature dependent magnetization M(T) and field dependent magnetization M(H) measurements. The results show that two phases including both ferromagnetic and superparamagnetic particles are present in our system. From these data we extracted the superparamagnetic critical size to be 9 nm for our samples. Ferromagnetic particles are single magnetic domain particles and the magnetic properties can be explained by the Stoner and Wohlfarth model. For the superparamagnetic phase, the effective anisotropy constant, K(eff), decreases as the particle size increases. (C)

2010 American Institute of Physics. [doi: 10.1063/1.3428415]“
“The LBH589 nmr differently sulfonated styrene-divinylbenzene cross-linked copolymer cationic exchange resins were prepared by oil-in-water polymerization and varied degrees of sulfonation. Several characteristics of the obtained resins were evaluated, i.e., Fourier transform infrared spectra, the ion-exchange capacity, microscopic morphology, size, and swelling. The resin characteristics were altered in relation to the degree of sulfonation, proving that differently sulfonated resins could be prepared. The behavior of chlorpheniramine (CPM) loading and in vitro release in the USP simulated gastric (SGF) and intestinal fluids (SIF) of the obtained resins were also evaluated.