Advanced patient age is not a contraindication for surgical treatment. Key Word(s): 1. advanced gastric cancer; 2. metastatic; 3. palliation; 4. surgery Presenting Author: XUEYUAN CAO Additional Authors: DONG HUI CAO, JING JIANG, TETSUYA TSUKAMOTO, MASAHIRO OSHIMA Corresponding Author: XUEYUAN CAO Affiliations: First Hospital of Jilin University, First Hospital of Jilin University, School of Medicine,
Fujita Health University, Kanazawa University Objective: 18β-Glycyrrhetinic acid (GRA), extracted from Liquorice root (Glycyrrhiza glabra), is known for its anti-tumor properties. And the anti-tumor properties might correlates with miRNA expression level, while the mechanism and target genes are not clear. K19-C2mE transgenic
(Tg) mice model could spontaneously develop the hyperplastic tumors in stomach. The purpose of this study was to systematically identify miRNAs correlated with hyperplastic tumor progression using K19-C2mE GSK126 concentration Tg mice model. Methods: K19-C2mE transgenic animal model of gastric tumor was established by Oshima M. Six-week-old K19-C2mE Tg mice were randomly divided into two groups: Control group (n = 40) and GRA-treated group ((n = 40, drinking water containing 0.05% GRA). After 52 weeks, total RNA enriched in miRNA samples were extracted from the tumors of Control group and GRA-treated group Caspase inhibitor clinical trial (mirVana™ miRNA Isolation Kit, ambion), reverse-trancribed (TaqMan® MicroRNA Reverse Transcription Kit) and assayed using Affymetrix GeneChip miRNA 3.0 Array. The incidence of gastric tumors was also detected. Results: The tumor incidence was decreased from 77.8% (28/36) to 33.4% (13/39) (P = 0.002) after GRA selleck inhibitor administration. MicroRNA array analysis found 30 miRNAs expression levels changed significantly (P 2.0), and 19 microRNAs were up-regulated and 11 miRNAs were down-regulated by GRA treatment. Two miRNAs correlated with tumor growth, MiRNA-128 and miRNA-30 were significantly down-regulated. And the abnormal
expression of miRNA-128 and miRNA-30 was correlated with Wnt/β-Catenin/BCL9 signaling pathway. Conclusion: 18β-Glycyrrhetinic acid could inhibit hyperplastic tumor growth and progression in K19-C2mE transgenic mice, and the inhibition effects might correlate with miRNA modulation. This work was supported by Norman Bethune Program of Jilin University [2013025], National Natural Science Foundation of China (81072369 and 81273065). Key Word(s): 1. miRNA-128; 2. miRNA-30; 3. 18ß-glycyrrhetinic acid; 4. gastric tumors; 5. transgenic mice Presenting Author: DONG HUI CAO Additional Authors: XUEYUAN CAO, JING JIANG, TETSUYA TSUKAMOTO, MASAHIRO OSHIMA Corresponding Author: XUEYUAN CAO Affiliations: First Hospital of Jilin University, First Hospital of Jilin University, School of Medicine, Fujita Health University, Kanazawa University Objective: Canolol (4-vinyl-2, 6-dimethoxyphenol), a natural antioxidant product, was shown anti-inflammatory and anti-tumor effects.