“
“Background: The aim of this study was to clarify the role of global hypomethylation of repetitive elements in determining the genetic and clinical features of multiple myeloma (MM).
Methods: We assessed global methylation levels using
four repetitive elements (long interspersed SN-38 clinical trial nuclear element-1 (LINE-1), Alu Ya5, Alu Yb8, and Satellite-a) in clinical samples comprising 74 MM samples and 11 benign control samples (7 cases of monoclonal gammopathy of undetermined significance (MGUS) and 4 samples of normal plasma cells (NPC)). We also evaluated copy-number alterations using array-based comparative genomic hybridization, and performed methyl-CpG binding domain sequencing (MBD-seq).
Results: Global levels of the repetitive-element methylation declined with the degree of malignancy of plasma cells (NPC>MGUS>MM), and there was a significant inverse correlation between the degree of genomic loss and the LINE-1 methylation levels. We identified 80 genomic loci as common breakpoints (CBPs) around commonly lost regions, which were significantly associated with increased LINE-1 densities. MBD-seq analysis revealed that average DNA-methylation levels at the CBP loci and relative methylation levels in regions with higher LINE-1 densities also declined during the development of MM. We confirmed that levels of methylation of the 5′ untranslated
region of respective LINE-1 loci correlated strongly with global LINE-1 methylation levels. Finally, there was a significant association between LINE-1 hypomethylation CYT387 clinical trial and poorer overall survival (hazard ratio 2.8, P = 0.015).
Conclusion: Global hypomethylation of LINE-1 MX69 in vitro is associated with the progression of and poorer prognosis for MM, possibly due to frequent copy-number loss.”
“Aromatic plant species of genus Thymus are important medicinal plants, highly recommended due to a range of therapeutic properties of their essential oils, commonly known as thyme oil:
antirheumatic, antiseptic, antispasmodic, antimicrobial, cardiac, carminative, diuretic and expectorant. The oil is also beneficial in boosting the immune system and helps to fight colds, flu, infectious diseases and chills. It is proved to be a urinary antiseptic, being very helpful for cystitis and urethritis. Scientific validation of traditional uses, and phytochemical and bioactivity evaluation of essential oils from Thymus serpyllum, Thymus algeriensis and Thymus vulgaris were performed.
GC/MS analysis revealed thymol as a major component of T. algeriensis, T. vulgaris and T. serpyllum, with its contribution to the oil 56.0%, 48.9% and 38.5%, respectively. All three essential oils (EOs) exhibited a significant antimicrobial activity against all tested strains, the T. serpyllum oil being the most potent one (MIC 2.