baujardi LPP7 at different stages and events of the life cycle of M. mayaguensis. M. mayaguensis is a very aggressive nematode that is destroying the guava industry in Brazil Chemical and cultural controls are providing adequate control ( Pereira et al., 2008). Biological control applying IJs of H. baujardi LPP7 to the soil to prevent the juveniles hatching was tested in the lab, however results were variable. This paper reports the selleck compound results dealing with embryogenesis and hatching of M. mayaguensis J2, when IJs of H. baujardi LPP7 are in contact. The IJs of H. baujardi LPP7 were reared

in larvae of Galleria mellonella L. (according to Woodring and Kaya, 1988), collected in modified White traps, and stored at 25 °C in a germination chamber for up to 7 days. The M. mayaguensis isolate was obtained from guava (Psidium guajava L.) in the municipality of São João da Barra, Brazil (lat. 21°39′21″ S; long. 41°2′7″ W), and it was maintained on tomato in pots with a mixture of autoclaved soil and river bed sand (1:1) in a greenhouse. To obtain eggs, small amounts of roots infected by nematodes were placed in 500 mL glass vials filled with 200 mL of tap water. The vials were shaken in a commercial shaker (TECNAL®, model TE240) for 4 min. The resulting

egg suspension was concentrated using a 150 μm sieve nested on a 25 μm learn more sieve (100 and 500 mesh, respectively) and used directly in the bioassays. Two treatments were compared: (i) embryogenesis of eggs in distilled water, and (ii) embryogenesis in distilled water in the presence of live IJs of H. baujardi LPP7. Each treatment consisted of 25 repetitions (eggs at the stage of two cells), which were distributed in five completely randomized blocks composed of Petri dishes with two glass slides that had a central cavity of 1 mL. In treatment 2, 10 IJs of H. baujardi LPP7 were added to each slide, and were replaced every

48 h. The slides were maintained in BOD at 25 °C for 336 h, completing the volume of water whenever necessary. The number of eggs with dead and alive embryos was evaluated at the end of the assay, as well as those which completed embryogenesis until the formation of J2. Living and dead embryos Verteporfin were differentiated through the incubation of eggs in an aqueous solution of phloxine B at 5% at room temperature for 30 min, observing the penetration of the dye only in eggs with dead embryos (Holbrook et al., 1983). The test was repeated once under the same conditions. Data was obtained and arcsine transformed and analyzed using analysis of variance (ANOVA) (SAEG, 1990). Differences in treatment means were separated using Tukey’s honestly significant difference procedure at P < 0.05. Two treatments were compared: (i) J2 hatching in distilled water and (ii) J2 hatching in distilled water in the presence of live IJs of H. baujardi LPP7.