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“The two B-cell non-Hodgkin’s lymphoma entities, chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL), show recurrent chromosomal gains of 3q25-q29, 12q13-q14 and 18q21-q22. The pathomechanisms affected by these aberrations are not understood. The aim of this study was to identify genes, located within these gained regions, which control cell death and cell survival of MCL and CLL cancer cells. Blood samples collected from 18 patients with CLL and 6 patients with MCL, as well as 6 cell lines representing both malignancies were analyzed by gene
expression profiling. By a comparison of genomic DNA and gene expression, 72 candidate find more genes were identified. We performed a limited RNA interference screening with these candidates to identify genes affecting cell survival. CCDC50 (coiled coil domain containing protein 50), SERPINI2 and SMARCC2 mediated a reduction of cell viability in primary CLL cells as well as Romidepsin chemical structure in cell lines. Gene knockdown and a nuclear factor kappa B (NF kappa B) reporter gene assay revealed that CCDC50 is required for
survival in MCL and CLL cells and controls NF kappa B signaling. Leukemia (2009) 23, 2018-2026; doi:10.1038/leu.2009.144; published online 30 July 2009″
“Palmitone is a secondary metabolite of polyketide origin extracted from leaves of Annona diversifolia Saff. (Annonaceae). We found that palmitone possesses anticonvulsant properties against penicillin-, 4-AP-, and Megestrol Acetate pentylenetetrazole (PTZ)-caused seizure in adult animals. Some convulsants as PTZ cause neuronal damage in different brain regions such as the CA3 hippocampal
region. Our objective was to evaluate if palmitone protects against M-caused seizures and hippocampal neuronal damage in prepubertal rats. We used 32 prepubertal Wistar rats (30-35 days old) divided into four groups of 8 animals; group I was the control group, group 11 received a single PTZ dose of 50 mg/kg ip, group III received a single palmitone dose of 50 mg/kg ip, and group IV received a palmitone dose of 50 mg/kg ip plus a PTZ dose of 50 mg/kg ip. Ten days after administration, the animals were killed using pentobarbital anesthesia (35 mg/kg). The brains were removed and were embedded in paraffin. Coronal cuts of 7 mu m were obtained from -2.8 to -3.3 from Bregma. Each section was stained with cresyl violet-eosin. We evaluated the number of normal and abnormal neurons in the CA3 hippocampal region in a 10,000 mu m(2) section. It was observed that palmitone did not prevent the PTZ-caused seizure but palmitone prevents the PTZ-caused neuronal damage in the CA3 hippocampal region. Published by Elsevier Ireland Ltd.”
“Alemtuzumab is active in chronic lymphocytic leukaemia (CLL) patients refractory to alkylators and fludarabine.