In a curing process, the hides are treated with sodium chloride and metam sodium. The salted hides are soaked to restore their natural humidity using a micro-biocide and enzymes. Hair removal/liming is done to remove the epidermis, hair and skin appendices. Hides are put in drums filled with lime, metam sodium as pesticide and sodium sulphide to achieve the alkaline

condition, which destroys the epidermal keratin. Hair and skin appendices are also removed manually with fleshing knives and a rotating knives H 89 mouse cylinder. In pre-tanning section, hides are undergone de-liming, bating and pickling. De-liming is done to remove excessive lime using hydrogen peroxide and carbon dioxide. Bating is the next step to remove excess hair using a protease enzyme and to remove natural fat (degreasing) using a lipase enzyme. Finally, the hide is transferred into an acid condition (pickling) using formic acid, Doramapimod manufacturer sulphuric acid,

sodium formate, sodium chloride and sodium metabisulphite. The skin of the worker is exposed to sodium chloride, sodium formate and sodium metabisulphite in this step. Sodium chloride may dehydrate the worker’s skin. Sodium metabisulphite is a skin sensitizer KPT330 (Kaaman et al. 2010; Madan et al. 2007; Sasseville and El-Helou 2009). Sodium chloride, sodium sulphide, soda ash, caustic soda, acetic Phospholipase D1 acid, formic acid and sulphuric acid have an irritant effect on the skin (NIOSH 2010; de Groot 2008). Metam sodium is a skin irritant (Koo et al. 1995) and contact sensitizer (Pruett et al. 2001). Tanning

stage Tanning is the chemical process to convert the hides into tanned leather by stabilizing the collagen structure, protecting the leather from enzymatic degradation, enhancing the strength and increasing its resistance to heat, hydrolysis and microbial degradation. Trivalent chromium sulphate is the most widely used tanning agent to form cross-linking collagen. Although our factories also performed vegetable tanning (using a mimosa wattle extract), they normally used potassium dichromate and phenosulphonic acid formaldehyde, together with mercaptobenzothiazole and metam sodium as a biocide.