Infect Immun 1986, 54:126–132.PubMed 20. de Haan CP, Kivistö R, Hänninen ML: Association of Campylobacter jejuni Cj0859c gene ( fspA ) variants with different C. jejuni multilocus sequence types . Appl Environ Microbiol 2010, 76:6942–6943.PubMedCrossRef 21. Kumar S, Nei M, Dudley J, Tamura

K: MEGA: a biologistcentric Software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 2008, 9:299–306.PubMedCrossRef 22. Jolley KA, Chan MS, Q-VD-Oph concentration Maiden MC: mlstdbNet-distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics 2004, 5:86.PubMedCrossRef Competing interests All authors declare no competing interests. Authors’ contributions AEZ conceived the study idea, performed all mathematical analysis and drafted the manuscript, Fosbretabulin in vitro CO performed Selleckchem CP690550 Bacterial culture,

DNA isolation and PCR-analysis, AMT performed DNA isolation and MLST-PCR, RL performed DNA sequencing and assisted in drafting the manuscript. UG participated in the study design and helped drafting the manuscript. All authors read, commented and approved the manuscript.”
“Background Polyketides are a large family of secondary metabolites with diverse structures and biological activities. Many of these are clinically important compounds with antibiotic, antifungal, and anticancer properties [1]. Polyketide biosynthesis ID-8 is catalyzed by a group of enzymes called polyketide synthases (PKSs). The carbon chain of polyketides is formed through stepwise decarboxylative condensation of acyl-thioester units by a coordinated group of PKS domains. The genes encoding PKS are usually clustered with their auxiliary and regulatory elements on the genome, and their products are classified into types I, II,

and III depending on their domain organization [2]. Bacterial aromatic polyketides such as tetracyclines and actinorhodin are polycyclic phenolic compounds that are assembled by type II PKSs. A characteristic of type II PKSs is domain composition with a maximum of 2 domains in each type II PKS and the iterative use of domains to synthesize a polyketide product [3]. Figure 1 shows the schematic diagram depicting the activity of type II PKS domains with actinorhodin biosynthesis as an example. Heterodimeric ketosynthase (KS) and chain length factor (CLF) domains catalyze chain initiation and elongation through decarboxylative condensation of malonyl building blocks, an acyl carrier protein (ACP) domain delivers malonyl building blocks to the KS-CLF, and a malonyl-CoA: ACP transacylase (MCAT) domain supplies malonyl groups to the ACP domain. The collective action of these type II PKS domains lead to the formation of highly reactive poly-β-keto intermediates.