J Bacteriol 2005, 187:8340–8349.PubMedCrossRef 37. van Opijnen T, Bodi KL, Camilli A: Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms. Nat Methods 2009, 6:767–772.PubMedCrossRef 38. Carvalho SM, Kloosterman TG, Kuipers OP, Neves AR: CcpA ensures optimal metabolic Anlotinib in vitro fitness of Streptococcus pneumoniae. PLoS One 2011, 6:e26707.PubMedCrossRef 39. Novichkov PS, Laikova ON, Novichkova ES, Gelfand MS, Arkin AP, Dubchak
I, et al.: RegPrecise: a database of curated genomic inferences of transcriptional regulatory interactions in prokaryotes. Nucleic Acids Res 2009, 38:D111-D118.PubMedCrossRef 40. Pearce BJ, Iannelli F, Pozzi G: Construction of new unencapsulated (rough) strains of Streptococcus pneumoniae. Res Microbiol 2002, 153:243–247.PubMedCrossRef 41. Pozzi G, Musmanno RA, Lievens PMJ, Oggioni MR, Plevani P, Manganelli R: Methods and parameters for genetic transformation of Streptococcus sanguis Challis. Res Microbiol 1990, 141:659–670.PubMedCrossRef 42. Pozzi G, Musmanno RA, Renzoni EA, Oggioni MR, Cusi MG: Host-vector system for integration of recombinant DNA into chromosomes of transformable and nontransformable streptococci.
J Bacteriol 1988, 170:1969–1972.PubMed 43. Iannelli F, Pozzi G: Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoniae. Mol Biotechnol 2004, 26:81–86.PubMedCrossRef 44. Chiavolini D, Memmi G, Maggi T, Iannelli F, Pozzi G, Oggioni MR: The three extra-cellular selleck zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice. Etofibrate BMC Microbiol 2003, 3:14.PubMedCrossRef 45. Carver T, GSK2399872A solubility dmso Beriman M, Tivey A, Patel C, Böhme U, Barrell BG, et al.: Artemis and ACT: viewing, annotating and comparing sequences stored in a relational database. Bioinformatics 2008, 24:2672–2676.PubMedCrossRef 46. Vickerman MM, Iobst S, Jesionowski AM, Gill SR: Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide.
J Bacteriol 2007, 189:7799–7807.PubMedCrossRef 47. Denapaite D, Bruckner R, Reichmann P, Henrich B, Maurer P, Schahle Y, et al.: The genome of Streptococcus mitis B6 – what is a commensal? PLoS One 2010, 5:e9426.PubMedCrossRef 48. Reichmann P, Nuhn M, Denapaite D, Bruckner R, Henrich B, Maurer P, et al.: Genome of Streptococcus oralis strain Uo5. J Bacteriol 2011, 193:2888–2889.PubMedCrossRef 49. Xu P, Alves JM, Kitten T, Brown A, Chen Z, Ozaki LS, et al.: Genome of the opportunistic pathogen Streptococcus sanguis. J Bacteriol 2007, 189:3166–3175.PubMedCrossRef 50. Oggioni MR, Iannelli F, Ricci S, Chiavolini D, Parigi R, Trappetti C, et al.: Antibacterial activity of a competence-stimulating peptide in experimental sepsis caused by Streptococcus pneumoniae. Antimicrob Agents Chemother 2004, 48:4725–4732.PubMedCrossRef 51.