The authors solve this conflict by showing that muricholic acid derivatives (TαMCA and TβMCA) act as FXR antagonists in both in vivo and in vitro experiments involving ileal stimulation with taurocholic acid. Thus, decreased Navitoclax solubility dmso ileal levels of MCA derivatives in CONV-R mice actually result in increased Fxr activation in the enterocyte due to the alleviation of βMCA-mediated

FXR-antagonism. The results of Sayin et al.[4] are significant since they demonstrate that GM influences BA homeostasis beyond the simple microbial metabolism, also acting as a direct regulator of CYP7A1 through the FGF15 pathway. Additionally, the regulation of the BA pool components by GM and the GM-induced shrinkage of BA pool may also have metabolic implications. Of note, recent findings by Watanabe et al.[10] suggest that a reduced BA pool size

may translate into reduced energy expenditure in brown adipose tissue, insulin resistance, and accumulation of triglycerides in the liver under high-fat-diet feeding conditions. Thus, it may be hypothesized that a larger MI-503 purchase BA pool present in GF mice may contribute to the reported resistance of these mice from diet-induced obesity.[1] In addition, metabolic implications could also result from deactivation of other nuclear receptors having BAs as physiological ligands such as vitamin D receptor, the pregnane-X-receptor, and the constitutive androstane receptor that play a role in a myriad of metabolic pathways. Another interesting finding is that βMCA is an FXR antagonist. Assuming that FXR antagonism is not beneficial to the hepatocyte, this

could represent a harmful side of hydrophilic BA therapy. In this line, FXR-antagonistic effects of very high ursodeoxycholic acid levels has been reported in humans.[11] On these grounds, one could speculate that FXR antagonism may be related to the increased risk of adverse outcomes in patients with primary sclerosing cholangitis treated with high doses of ursodeoxycholic acid. Finally, it should be kept in mind that these findings cannot be directly extrapolated to humans, since mice and men exhibit multiple differences in biliary physiology.[12, 13] For 上海皓元医药股份有限公司 example, with regard to bile acid pool composition, MCA and its derivatives are exclusively present in rodents, while in humans the BA pool is predominantly constituted by CA. Also, some enzymatic pathways such as rehydroxylation of secondary BAs like deoxycholic acid, which results in its conversion to CA, are not present in human beings. These differences determine marked variations in BA pool hydrophyllicity between both species and may have importance in their response in pathological settings such as cholestasis and increased levels of DCA, since the latter is a powerful activator of a myriad specific cell signaling pathways and receptors (i.e., EGFR, protein kinase C, β-catenin) with potential effects on cells of the EHC.