To commemorate the interesting work carried out by the FEBS Long-Term Fellows, we provide here a special ‘In the Limelight’ dilemma of FEBS Open Bio, containing four Mini-reviews and four Research Protocols authored because of the fellows on their own. The four Review articles supply timely updates on the respective research fields, even though the Research Protocols explain simple tips to perform challenging experimental practices in detail. We wish this issue will be an invaluable resource for the neighborhood, and a celebration regarding the top-notch work carried out by young scientists.Circadian rhythms coordinate biological processes with world’s 24-h daily light/dark pattern. In the last many years, attempts in the area of chronobiology have actually tried to know the methods when the circadian clock settings transcription across areas and cells. It has been supported by the development of various bioinformatic techniques that allow the recognition of 24-h oscillating transcripts. This workflow aims to describe how to separate muscle mass stem cells for RNA sequencing evaluation from a typical circadian experiment and presents bioinformatic resources ideal for the analysis of circadian transcriptomes.Ulcerative colitis (UC) is an inflammatory infection for the large intestine that is described as diarrhoea, bloody stools, abdominal pain and mucosal ulceration. UC is treated with nonsteroidal anti inflammatory medications, corticosteroids or immunosuppressants, but long-term use of these medications trigger negative effects. Arum maculatum is employed as a normal treatment plan for gastrointestinal system disorders, but its use for treatment of UC is not Nutlin3a examined rigorously. We investigated the possible protective aftereffect of a methanol plant of A. maculatum against dextran sulfate sodium (DSS) caused experimental UC in rats. Total phenolic and flavonoid articles associated with the extract had been 32.919 ± 1.125 mg gallic acid equivalent (GAE)/g and 52.045 ± 7.902 µg rutin equivalent (RE)/mg, respectively. The half-maximal inhibitory concentration (IC50) for the herb ended up being 105.76 µg/ml according into the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging task assay. Results of A. maculatum plant on UC induced by DSS had been evaluated both macroscopically and histologically. We also investigated effects of A. maculatum extract on malondialdehyde (MDA) levels additionally the oxidative anxiety index (OSI) in typical rats and rats with UC. We unearthed that therapy with A. maculatum herb safeguarded the colon against DSS induced UC in a dose-dependent way.Highly contagious breathing ailments like influenza and COVID-19 pose severe risks to general public wellness. A two-in-one vaccine is ideal in order to avoid numerous vaccinations for those diseases. Right here, we produced a chimeric receptor binding domain of the spike protein (S-RBD) and hemagglutinin (HA)-stalk-based vaccine both for SARS-CoV-2 and influenza viruses. The S-RBD from SARS-CoV-2 Delta had been fused to the headless HA from H1N1 (H1Delta), creating a chimera that types trimers in answer. The cryo-electron microscopy structure associated with chimeric necessary protein complexed utilizing the RBD-targeting CB6 and the HA-stalk-targeting CR9114 antibodies shows that the trimeric protein is steady and available for neutralizing antibody binding. Immunization because of the vaccine elicited high and durable neutralizing antibodies and effectively protected mice resistant to the challenges of lethal H1N1 or heterosubtypic H5N8, as well since the SARS-CoV-2 Delta or Omicron BA.2 alternatives. Overall, this study offers a two-in-one universal vaccine design to combat infections caused by both SARS-CoV-2 alternatives of issue and influenza viruses. Recent technologies and brand new devices are developed in vitreoretinal surgeries, plus they supply additional information, enhance protection, improve surgeons’ comfort, and better visual and anatomical results. Some products being useful for better visualization during surgery, plus some help the operation overall performance. They have been divided in to listed here titles Intraoperative OCT (including hand-held, probe-integrated, and microscope-integrated OCT), three-dimensional visualization system, digital truth system, endoscopic vitrectomy (fibre optics and non-fiber optics), wide-angle watching methods (contact and non-contact contacts), endo-illumination, light filters, chromovitrectomy, the retinal prosthesis (including epiretinal, subretinal, and suprachoroidal products), robot-assisted vitreoretinal surgery, more recent Resting-state EEG biomarkers Vitreoretinal devices, gene and cellular therapy microfluidic biochips . The key purpose of this analysis would be to upgrade your reader from the latest development in intraoperative imaging and surgical vision technologies and also to provide an awareness of just how each has actually helped improve procedure and surgical effects. The surgeons should be aware recent revisions to accomplish their best and attain the absolute most positive results.The primary purpose of this analysis is always to upgrade the reader on the latest progression in intraoperative imaging and medical vision technologies and also to offer an awareness of how each has actually helped improve procedure and medical results. The surgeons ought to know recent changes to do their best and attain probably the most very good results. Between December 1 and 31, 2022, we searched for the English version of published research reports on public attitude toward epilepsy in Ethiopia in PubMed/Medline, Science Direct, Cochrane Library, Google Scholar, and PsycINFO. The study reports’ quality ended up being assessed making use of the Newcastle-Ottawa Scale. We extracted the relevant information through the searched papers in a Microsoft Excel format and imported it to STATA version 15.0, for evaluation.