58% and 26 02% for proteome and metabolism However, no epistasis

58% and 26.02% for proteome and metabolism. However, no epistasis was detected for genome and transcriptome loci. In contrast, the proportions of epistasis and treatment interaction effects on heritability Thiazovivin in vivo (hqe + qqe2) were 51.65%, but only 0.70% and 3.84% at the transcriptome, proteome and metabolome levels, respectively. Molecular markers

have enormous potential to improve the efficiency and precision of conventional plant breeding via marker-assisted selection [29]. The important challenge of applying genetic and -omics data to breeding is the identification of the genes underlying a trait of interest. We performed an integrated association mapping for chromium content and total sugar content based on genome, transcriptome, proteome and metabolites, and detected some QTSs, QTTs, QTPs and QTMs associated with two complex traits. The strategy to employ these molecular loci in the breeding practice should be considered prudently. For example, those QTX based on methylated loci of the genome were essentially directly useful as DNA markers and would be directly applicable in breeding practice. In terms of marker assisted breeding for each of the two traits, chromium content could be selected with Phm1376 which had a

significant positive additive effect (− log10P = 10.05, and hq2 = 20.29), indicating that demethylation of this locus could reduce chromium content for three varieties in two locations. The qe (additive by treatment interaction) effect of Phm1376 was negative in Guiding for all three varieties tested www.selleckchem.com/products/dabrafenib-gsk2118436.html in this study, but in Xingyi they were positive. This suggests that reduction of chromium content in tobacco leaves could be achieved by methylation of this locus in

Guiding but demethylation of the same locus in Xingyi. The epistasis of Phm1053 and Phm1471 only had qqe effects in Xingyi, positive for K326 and Hongda, but negative for Zunyan 6, indicating that the best genotypic state in the two loci was demethylation for varieties K326 and Hongda, but methylation for Zunyan 6. In the cases of the QTTs and QTPs associated with Phospholipase D1 the traits of interest, there might be two strategies to use in practical plant breeding. For the first strategy, bioinformatics analysis can be carried out to make sure that the function of the transcript or proteins is based on a functional gene association with the investigated traits, and then the representative gene of the transcript can be developed as a DNA based marker useful for marker-assisted-selection. This strategy is more valuable when the transcripts or proteins have large genetic effects and high heritability. The second strategy would be based on direct use of the transcript as an indicative marker, where the abundance of the transcript would predict the performance of the genotype for the traits. In this study, two transcripts and two miRNAs presented association with chromium content.

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