Despite wide expression and involvement in multiple pathological

Despite wide expression and involvement in multiple pathological conditions, the lack of OPN in mice is not embryonically lethal nor does it

causes a prominent phenotype compared to wild type mice suggesting that alternative mechanisms compensate for the lack of OPN or it may not play a key role in embryonic development [44]. One of the main challenges in characterizing role of OPN in tumor progression is the existence of two distinct families of receptors including integrins and CD44v6 Androgen Receptor Antagonist that have the capacity to trigger downstream signaling pathways independent of each other. Therefore, inhibition of one of the two receptors/pathways may not completely suppress OPN signalling and development of learn more therapeutic compounds to inhibit both receptors is extremely challenging if not impossible. In the tumor mass, OPN is secreted by both stroma and cancer cells [36]. It appears that there are distinct functions for tumor-derived vs. stromal-derived OPN in tumor growth and metastasis. Crawford et al developed a model of cutaneous squamous cell carcinoma in OPN null mice and showed that while the number of metastatic tumors is increased CX-6258 mouse in this model, the size of metastasized tumors was significantly lower

compared to corresponding wild type mice [45]. It is suggested that stromal OPN may recruit anti-tumor macrophages resulting in smaller tumor growth [45]. However, other reports in melanoma [46] and breast [47] tumors suggest that host-derived OPN is important r for tumor growth and metastasis adding to the complexity of OPN in tumor biology. Here, we developed an anti-OPN antibody capable of neutralizing human and mouse OPN, and utilized it to investigate the role of OPN in preclinical models with particular focus on lung cancer since a significant amount of data supports a role for OPN in NSCLCs [48]. All three transcripts of OPN have been identified in NSCLC patients

and gain-of-function analyses indicate that OPNa, but not OPNb or OPNc, is involved in increased proliferation, migration, and invasion of tumor cells [49]. Serum OPN has been shown to act as a biomarker in lung carcinoma [38, 50]. Conversely, reduction in serum OPN (e.g. due to resection of primary Decitabine concentration tumors) [51] is an indicator of better outcome in NSCLC patients treated with cytotoxic agent [52]. Despite all these reports, it remains to be clearly determined if OPN is a biomarker and/or a driver of tumor progression in NSCLC. The KrasG12D-LSLp53fl/fl mice [53] is one of the most relevant preclinical models of NSCLC since 20-30% of NSCLC patients carry Kras mutation [54] and 35-60% show genetic aberrations in p53 [55]. Capacity of tumor fragments to engraft in immuno-deficient animals provided an opportunity to test efficacy of AOM1 in NSCLC tumors. Lack of response to AOM1 in primary tumor growth indicates an overlapping mechanism between OPN and the other tumor-promoting factors.

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